![]() The Kinetex column appears to have a better load. I may have overloaded the Supelco column, giving a lower plate count and more asymmetry) Conclusion: The Kinetex and Supelco columns are probably comparable. " Direct comparison of Supelco Discovery C18 (5 µm, 150x4.6mm) and Kinetex C18 (5 µm, 150 x 4.6mm): Analysed the same day on the same instrument (Agilent 1200), using the same sample and mobile phase: Instrument: Agilent 1200 Mobile phase: 20% acetonitrile in 5 mM acetate buffer pH 4.5 (isocratic) Sample: 20 µl of a 100 µg/ml solution (in-house peptide) Flow: 0.5 ml/min Temp: 50☌ Retention time Supelco column: 18 minutes Kinetex column: 14 minutes (More retention on the Supelco column, maybe not so surprising) Tailing factor Supelco column: 1.8 Kinetex column: 1.4 (clearly better symmetry on the Kinetex column) Plate count Supelco column: 12.000 plates Kinetex column: 16.000 plates (I am a bit shocked that the Kinetex column gave so much better efficiency. In comparison studies with other C18 columns, this new Kinetex phase has produced differences in peak elution order and improved separation of co-eluting. Review date: | Kinetex Core-Shell HPLC/UHPLC Columns Their customer care and technical support teams are very " But the current column has survived over 500 tissue samples (blend and organic solvent precipitation) without notable degradation. Designed to maximize performance of HPLC/UHPLC systems by maximizing efficiency, resulting in enhanced. We haven't used those for long enough to comment on their longevity. Features of Navigatorsil Core-Shell Columns. The new EVO columns give the same or better peak shape, with significantly wider usable pH range. They are well suited for a simple drop-in upgrade for older instruments that cannot handle the high pressure of small particle size columns. The 5 micron columns give 70~80% of the resolution power of what 2.7 micron columns offer, with significantly lower back pressure. Some of our samples have very polar and chelating bisphosphonate groups, and the column handled them well. The column survived well and gave excellent results, both in peak shape and sharpness. Most of the time it's a simple dip-dilute-and-shoot sample prep with no specific clean-up. ![]() " We use Kinetex 5 micron C18 columns in a synthetic lab for routine reaction monitoring.
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